Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 92901..93424 | Replicon | plasmid p2GR390 |
| Accession | NZ_CP075892 | ||
| Organism | Klebsiella pneumoniae strain GR390 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | V5RNQ6 |
| Locus tag | J4H96_RS28115 | Protein ID | WP_015632464.1 |
| Coordinates | 92901..93206 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | V5RQR8 |
| Locus tag | J4H96_RS28120 | Protein ID | WP_015632465.1 |
| Coordinates | 93206..93424 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J4H96_RS28070 (J4H96_28070) | 87931..88359 | - | 429 | WP_015632456.1 | plasmid partitioning/stability family protein | - |
| J4H96_RS28075 (J4H96_28075) | 88352..89332 | - | 981 | WP_015632457.1 | plasmid segregation protein ParM | - |
| J4H96_RS28080 (J4H96_28080) | 89693..89875 | + | 183 | WP_015632458.1 | type II toxin-antitoxin system HicA family toxin | - |
| J4H96_RS28085 (J4H96_28085) | 89901..90344 | + | 444 | WP_015632459.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| J4H96_RS28090 (J4H96_28090) | 90550..90672 | - | 123 | Protein_98 | transcriptional regulator | - |
| J4H96_RS28095 (J4H96_28095) | 90686..90913 | - | 228 | WP_016162072.1 | hypothetical protein | - |
| J4H96_RS28970 | 91145..91600 | + | 456 | WP_015632461.1 | helix-turn-helix domain-containing protein | - |
| J4H96_RS28975 | 91852..91947 | - | 96 | Protein_101 | IS1 family transposase | - |
| J4H96_RS28110 (J4H96_28110) | 92412..92789 | - | 378 | WP_016162069.1 | hypothetical protein | - |
| J4H96_RS28115 (J4H96_28115) | 92901..93206 | - | 306 | WP_015632464.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| J4H96_RS28120 (J4H96_28120) | 93206..93424 | - | 219 | WP_015632465.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| J4H96_RS28125 (J4H96_28125) | 93610..94635 | + | 1026 | WP_015632466.1 | IS110 family transposase | - |
| J4H96_RS28130 (J4H96_28130) | 94930..95415 | + | 486 | Protein_106 | IS5 family transposase | - |
| J4H96_RS28135 (J4H96_28135) | 95579..96010 | + | 432 | WP_015632467.1 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
| J4H96_RS28140 (J4H96_28140) | 96010..97281 | + | 1272 | WP_016162068.1 | Y-family DNA polymerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aac(6')-Ib / blaNDM-1 | - | 1..97674 | 97674 | |
| - | flank | IS/Tn | - | - | 93610..94635 | 1025 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11361.90 Da Isoelectric Point: 5.5252
>T204491 WP_015632464.1 NZ_CP075892:c93206-92901 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI
Download Length: 306 bp
>T204491 NZ_CP100739:2357177-2357461 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8194.13 Da Isoelectric Point: 6.2224
>AT204491 WP_015632465.1 NZ_CP075892:c93424-93206 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW
Download Length: 219 bp
>AT204491 NZ_CP100739:2356939-2357187 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6C0ND19 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6C0L1T7 |