Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6246..6889 Replicon plasmid p2GR390
Accession NZ_CP075892
Organism Klebsiella pneumoniae strain GR390

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag J4H96_RS27655 Protein ID WP_001044770.1
Coordinates 6473..6889 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J4H96_RS27650 Protein ID WP_001261282.1
Coordinates 6246..6476 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4H96_RS27610 (J4H96_27610) 1527..2153 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
J4H96_RS28960 2150..2452 + 303 WP_004197636.1 hypothetical protein -
J4H96_RS27620 (J4H96_27620) 2948..3742 - 795 WP_015632375.1 site-specific integrase -
J4H96_RS27625 (J4H96_27625) 3735..3896 - 162 WP_032495756.1 hypothetical protein -
J4H96_RS27630 (J4H96_27630) 3940..4944 - 1005 WP_016162067.1 hypothetical protein -
J4H96_RS27635 (J4H96_27635) 5009..5320 - 312 WP_024191724.1 hypothetical protein -
J4H96_RS27640 (J4H96_27640) 5369..5683 - 315 WP_015632378.1 hypothetical protein -
J4H96_RS27645 (J4H96_27645) 5828..6289 - 462 WP_165763053.1 hypothetical protein -
J4H96_RS27650 (J4H96_27650) 6246..6476 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J4H96_RS27655 (J4H96_27655) 6473..6889 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
J4H96_RS27660 (J4H96_27660) 6963..7652 + 690 Protein_11 AAA family ATPase -
J4H96_RS27665 (J4H96_27665) 7707..8404 + 698 WP_223174630.1 IS1 family transposase -
J4H96_RS27670 (J4H96_27670) 8421..8612 - 192 Protein_13 nucleotidyltransferase domain-containing protein -
J4H96_RS27675 (J4H96_27675) 8682..9236 - 555 WP_063840280.1 AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase -
J4H96_RS27680 (J4H96_27680) 9470..10027 - 558 WP_001217881.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / blaNDM-1 - 1..97674 97674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T204490 WP_001044770.1 NZ_CP075892:6473-6889 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T204490 NZ_CP100739:2014370-2014473 [Salmonella enterica subsp. enterica serovar Typhimurium]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT204490 WP_001261282.1 NZ_CP075892:6246-6476 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT204490 NZ_CP100739:c2014475-2014330 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References