Detailed information of TA system TA020449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2849754..2850394
• Replicon: chromosome
• Accession: NC_002755
• Organism: Mycobacterium tuberculosis CDC1551

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: MT_RS13300
• Protein ID: WP_003412970.1
• Coordinates: 2849754..2850173 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: MT_RS13305
• Protein ID: WP_003412975.1
• Coordinates: 2850170..2850394 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT_RS13275	2845340..2846062	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
MT_RS13280	2846578..2846805	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
MT_RS13285	2846802..2847203	+	402	WP_003412963.1	PIN domain-containing protein	-
MT_RS13290	2847238..2848158	-	921	WP_003412965.1	restriction endonuclease	-
MT_RS22400	2848499..2848744	-	246	WP_162466973.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MT_RS13295	2848803..2849753	+	951	WP_003911916.1	Lsr2 family protein	-
MT_RS13300	2849754..2850173	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
MT_RS13305	2850170..2850394	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
MT_RS13310	2850425..2853268	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
MT_RS22645	2853340..2853741	-	402	WP_003412981.1	hypothetical protein	-
MT_RS22650	2853784..2854212	-	429	Protein_2608	transcription antitermination factor NusB	-
MT_RS13325	2854215..2854778	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T20449 WP_003412970.1 NC_002755:c2850173-2849754 [Mycobacterium tuberculosis CDC1551]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T20449 NC_002755:c2850173-2849754 [Mycobacterium tuberculosis CDC1551]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT20449 WP_003412975.1 NC_002755:c2850394-2850170 [Mycobacterium tuberculosis CDC1551]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT20449 NC_002755:c2850394-2850170 [Mycobacterium tuberculosis CDC1551]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09