Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 96108..96844 Replicon plasmid p1GR390
Accession NZ_CP075891
Organism Klebsiella pneumoniae strain GR390

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag J4H96_RS27130 Protein ID WP_003026803.1
Coordinates 96108..96590 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag J4H96_RS27135 Protein ID WP_003026799.1
Coordinates 96578..96844 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4H96_RS27110 (J4H96_27110) 91462..93225 - 1764 WP_004200842.1 DUF262 domain-containing protein -
J4H96_RS27115 (J4H96_27115) 93426..93617 - 192 Protein_85 helix-turn-helix domain-containing protein -
J4H96_RS27120 (J4H96_27120) 93843..94475 + 633 WP_001567369.1 hypothetical protein -
J4H96_RS27125 (J4H96_27125) 94504..95907 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
J4H96_RS27130 (J4H96_27130) 96108..96590 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
J4H96_RS27135 (J4H96_27135) 96578..96844 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
J4H96_RS27140 (J4H96_27140) 97089..97523 - 435 WP_001567367.1 cell envelope integrity protein TolA -
J4H96_RS27145 (J4H96_27145) 97570..98118 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
J4H96_RS27150 (J4H96_27150) 98570..98920 + 351 WP_004200891.1 hypothetical protein -
J4H96_RS27155 (J4H96_27155) 99819..101357 - 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
J4H96_RS27160 (J4H96_27160) 101406..101753 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / tet(A) - 1..187495 187495
- inside IScluster/Tn - - 94504..102387 7883


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T204489 WP_003026803.1 NZ_CP075891:c96590-96108 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T204489 NZ_CP100739:c995295-994768 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT204489 WP_003026799.1 NZ_CP075891:c96844-96578 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT204489 NZ_CP100739:c995582-995292 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References