Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5356224..5356849 Replicon chromosome
Accession NZ_CP075890
Organism Klebsiella pneumoniae strain GR390

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J4H96_RS26330 Protein ID WP_002882817.1
Coordinates 5356224..5356607 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J4H96_RS26335 Protein ID WP_004150355.1
Coordinates 5356607..5356849 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4H96_RS26315 (J4H96_26315) 5353590..5354492 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J4H96_RS26320 (J4H96_26320) 5354489..5355124 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J4H96_RS26325 (J4H96_26325) 5355121..5356050 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J4H96_RS26330 (J4H96_26330) 5356224..5356607 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J4H96_RS26335 (J4H96_26335) 5356607..5356849 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J4H96_RS26340 (J4H96_26340) 5357054..5357971 + 918 WP_002882812.1 alpha/beta hydrolase -
J4H96_RS26345 (J4H96_26345) 5357985..5358926 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J4H96_RS26350 (J4H96_26350) 5358971..5359408 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J4H96_RS26355 (J4H96_26355) 5359405..5360265 - 861 WP_002882807.1 virulence factor BrkB family protein -
J4H96_RS26360 (J4H96_26360) 5360259..5360858 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T204488 WP_002882817.1 NZ_CP075890:c5356607-5356224 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T204488 NZ_CP100739:849416-849814 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT204488 WP_004150355.1 NZ_CP075890:c5356849-5356607 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT204488 NZ_CP100739:849189-849416 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References