Detailed information of TA system TA204469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4246017..4247605
• Replicon: chromosome
• Accession: NZ_CP075885
• Organism: Klebsiella quasipneumoniae strain JNQH473

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KLH66_RS21760
• Protein ID: WP_101990367.1
• Coordinates: 4246017..4247339 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: KLH66_RS21765
• Protein ID: WP_023289667.1
• Coordinates: 4247339..4247605 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KLH66_RS21745 (4241049)	4241049..4243574	+	2526	WP_214632726.1	fimbria/pilus outer membrane usher protein	-
KLH66_RS21750 (4243562)	4243562..4244566	+	1005	WP_130952623.1	fimbrial protein	-
KLH66_RS21755 (4244708)	4244708..4246000	+	1293	WP_044523014.1	glycoside hydrolase family 10 protein	-
KLH66_RS21760 (4246017)	4246017..4247339	-	1323	WP_101990367.1	type II toxin-antitoxin system HipA family toxin	Toxin
KLH66_RS21765 (4247339)	4247339..4247605	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KLH66_RS21770 (4247926)	4247926..4248252	-	327	WP_044523006.1	hydroxyisourate hydrolase	-
KLH66_RS21775 (4248249)	4248249..4248749	-	501	WP_032452825.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KLH66_RS21780 (4248727)	4248727..4250106	-	1380	WP_065812518.1	nucleobase:cation symporter-2 family protein	-
KLH66_RS21785 (4250452)	4250452..4251606	+	1155	WP_214632727.1	FAD-dependent urate hydroxylase HpxO	-
KLH66_RS21790 (4251587)	4251587..4252510	-	924	WP_214632728.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49239.60 Da
• Isoelectric Point: 8.9176

>T204469 WP_101990367.1 NZ_CP075885:c4247339-4246017 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPVDFSAHVWRAITENMLKLHARLRQGLQAR

Nucleotide

• Length: 1323 bp

>T204469 NZ_CP100736:221339-221824 [Salmonella enterica subsp. enterica serovar Agona]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTTGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT204469 WP_023289667.1 NZ_CP075885:c4247605-4247339 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT204469 NZ_CP100736:221064-221351 [Salmonella enterica subsp. enterica serovar Agona]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTATATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3