Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 14159..14895 Replicon plasmid pJNQH473-1
Accession NZ_CP075884
Organism Klebsiella quasipneumoniae strain JNQH473

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KLH66_RS00100 Protein ID WP_003026803.1
Coordinates 14413..14895 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KLH66_RS00095 Protein ID WP_003026799.1
Coordinates 14159..14425 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KLH66_RS00060 (KLH66_00060) 10003..10365 - 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -
KLH66_RS00065 (KLH66_00065) 10417..10767 - 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KLH66_RS00070 (KLH66_00070) 11125..11373 + 249 WP_004193994.1 hypothetical protein -
KLH66_RS00075 (KLH66_00075) 11370..12581 + 1212 WP_032415728.1 hypothetical protein -
KLH66_RS00080 (KLH66_00080) 12715..13206 + 492 WP_004206662.1 hypothetical protein -
KLH66_RS00085 (KLH66_00085) 13267..13470 + 204 WP_004206661.1 HHA domain-containing protein -
KLH66_RS00090 (KLH66_00090) 13484..13714 + 231 WP_004206660.1 hypothetical protein -
KLH66_RS00095 (KLH66_00095) 14159..14425 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KLH66_RS00100 (KLH66_00100) 14413..14895 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KLH66_RS00105 (KLH66_00105) 15096..16499 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
KLH66_RS00110 (KLH66_00110) 16528..17160 - 633 WP_001567369.1 hypothetical protein -
KLH66_RS00115 (KLH66_00115) 17389..18735 + 1347 WP_077251107.1 ISNCY family transposase -
KLH66_RS00120 (KLH66_00120) 18895..19599 + 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-14 / blaIMP-8 mrkA / mrkB / mrkC / mrkF / mrkJ 1..229265 229265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T204457 WP_003026803.1 NZ_CP075884:14413-14895 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T204457 NZ_CP100732:2014369-2014472 [Salmonella enterica subsp. enterica serovar Typhimurium]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT204457 WP_003026799.1 NZ_CP075884:14159-14425 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT204457 NZ_CP100732:c2014474-2014329 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References