Detailed information of TA system TA204455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 172107..173017
• Replicon: plasmid pJNQH491-1
• Accession: NZ_CP075882
• Organism: Klebsiella michiganensis strain JNQH491

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: KLH67_RS29370
• Protein ID: WP_004144067.1
• Coordinates: 172547..173017 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6A8PHD0
• Locus tag: KLH67_RS29365
• Protein ID: WP_023287184.1
• Coordinates: 172107..172550 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KLH67_RS29345 (KLH67_29340)	167523..168272	+	750	Protein_170	IS5 family transposase	-
KLH67_RS29350 (KLH67_29345)	168301..169269	+	969	WP_074424072.1	IS5 family transposase	-
KLH67_RS29355 (KLH67_29350)	169465..170811	+	1347	WP_225517490.1	ThiF family adenylyltransferase	-
KLH67_RS29360 (KLH67_29355)	170864..172006	+	1143	Protein_173	translesion error-prone DNA polymerase V subunit UmuC	-
KLH67_RS29365 (KLH67_29360)	172107..172550	+	444	WP_023287184.1	DUF2384 domain-containing protein	Antitoxin
KLH67_RS29370 (KLH67_29365)	172547..173017	+	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
KLH67_RS29375 (KLH67_29370)	173104..174099	+	996	WP_077254794.1	IS5 family transposase	-
KLH67_RS29380 (KLH67_29375)	174101..175069	+	969	WP_072096747.1	IS5 family transposase	-
KLH67_RS29385 (KLH67_29380)	175258..175518	-	261	WP_017901409.1	hypothetical protein	-
KLH67_RS29390 (KLH67_29385)	175823..176791	+	969	WP_074423483.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..205582	205582
	-	inside	IScluster/Tn	-	-	165379..176791	11412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T204455 WP_004144067.1 NZ_CP075882:172547-173017 [Klebsiella michiganensis]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T204455 NZ_CP100732:849416-849814 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16522.84 Da
• Isoelectric Point: 10.3013

>AT204455 WP_023287184.1 NZ_CP075882:172107-172550 [Klebsiella michiganensis]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT204455 NZ_CP100732:849189-849416 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: A0A6A8PHD0