204454

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35635..36287 Replicon plasmid pJNQH491-1
Accession NZ_CP075882
Organism Klebsiella michiganensis strain JNQH491

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag KLH67_RS28685 Protein ID WP_017901321.1
Coordinates 35635..36060 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag KLH67_RS28690 Protein ID WP_001261275.1
Coordinates 36057..36287 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KLH67_RS28660 (KLH67_28655) 31344..31460 + 117 Protein_31 transposase -
KLH67_RS31390 31585..31755 - 171 Protein_32 LysR family transcriptional regulator -
KLH67_RS31395 31766..31993 + 228 Protein_33 IS3 family transposase -
KLH67_RS28670 (KLH67_28665) 32085..33641 - 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
KLH67_RS31400 33828..34001 - 174 Protein_35 nuclease -
KLH67_RS28675 (KLH67_28670) 34054..34590 - 537 Protein_36 integrase core domain-containing protein -
KLH67_RS28680 (KLH67_28675) 34649..35617 - 969 WP_077254762.1 IS5 family transposase -
KLH67_RS28685 (KLH67_28680) 35635..36060 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
KLH67_RS28690 (KLH67_28685) 36057..36287 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KLH67_RS28695 (KLH67_28690) 36540..39116 - 2577 WP_017901322.1 nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..205582 205582


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T204454 WP_017901321.1 NZ_CP075882:c36060-35635 [Klebsiella michiganensis]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T204454 NZ_CP100732:838549-838962 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT204454 WP_001261275.1 NZ_CP075882:c36287-36057 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT204454 NZ_CP100732:838302-838568 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References