Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 5707417..5708036 Replicon chromosome
Accession NZ_CP075881
Organism Klebsiella michiganensis strain JNQH491

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag KLH67_RS26965 Protein ID WP_004099646.1
Coordinates 5707818..5708036 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag KLH67_RS26960 Protein ID WP_004129911.1
Coordinates 5707417..5707791 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KLH67_RS26950 (KLH67_26945) 5702574..5703767 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KLH67_RS26955 (KLH67_26950) 5703790..5706936 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
KLH67_RS26960 (KLH67_26955) 5707417..5707791 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
KLH67_RS26965 (KLH67_26960) 5707818..5708036 + 219 WP_004099646.1 HHA domain-containing protein Toxin
KLH67_RS26970 (KLH67_26965) 5708199..5708765 + 567 WP_038424164.1 maltose O-acetyltransferase -
KLH67_RS31365 5708737..5708871 - 135 WP_223226764.1 hypothetical protein -
KLH67_RS26975 (KLH67_26970) 5708892..5709362 + 471 WP_014228205.1 YlaC family protein -
KLH67_RS26980 (KLH67_26975) 5709337..5710791 - 1455 WP_042944459.1 PLP-dependent aminotransferase family protein -
KLH67_RS26985 (KLH67_26980) 5710893..5711591 + 699 WP_014228203.1 GNAT family protein -
KLH67_RS26990 (KLH67_26985) 5711588..5711728 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KLH67_RS26995 (KLH67_26990) 5711728..5711991 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T204452 WP_004099646.1 NZ_CP075881:5707818-5708036 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T204452 NZ_CP100732:316726-317094 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT204452 WP_004129911.1 NZ_CP075881:5707417-5707791 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT204452 NZ_CP100732:316508-316729 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References