Detailed information of TA system TA204443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3054687..3055277
• Replicon: chromosome
• Accession: NZ_CP075881
• Organism: Klebsiella michiganensis strain JNQH491

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KLH67_RS14350
• Protein ID: WP_046878085.1
• Coordinates: 3054945..3055277 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J6I3K7
• Locus tag: KLH67_RS14345
• Protein ID: WP_004852307.1
• Coordinates: 3054687..3054944 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KLH67_RS14325 (KLH67_14325)	3049936..3050541	-	606	WP_049084130.1	glutathione S-transferase family protein	-
KLH67_RS14330 (KLH67_14330)	3050721..3051647	+	927	WP_049084131.1	LysR substrate-binding domain-containing protein	-
KLH67_RS14335 (KLH67_14335)	3051685..3052683	-	999	WP_049084133.1	aldo/keto reductase	-
KLH67_RS14340 (KLH67_14340)	3052784..3053698	+	915	WP_049084134.1	LysR family transcriptional regulator	-
KLH67_RS31220	3053860..3053976	-	117	Protein_2796	molybdopterin-binding protein	-
KLH67_RS14345 (KLH67_14345)	3054687..3054944	+	258	WP_004852307.1	antitoxin	Antitoxin
KLH67_RS14350 (KLH67_14350)	3054945..3055277	+	333	WP_046878085.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KLH67_RS14355 (KLH67_14355)	3055709..3056896	+	1188	WP_045338339.1	nucleoid-associated protein	-
KLH67_RS14360 (KLH67_14360)	3056914..3058077	+	1164	WP_152403861.1	hypothetical protein	-
KLH67_RS14365 (KLH67_14365)	3058482..3059231	-	750	Protein_2801	integrase core domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11884.86 Da
• Isoelectric Point: 10.8850

>T204443 WP_046878085.1 NZ_CP075881:3054945-3055277 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTLGVIRCDQPRTI
DIAARNGKRLERIPDAVVNEVLARLDAILR

Nucleotide

• Length: 333 bp

>T204443 NZ_CP100728:3548363-3548581 [Salmonella enterica subsp. enterica serovar Blockley]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9422.88 Da
• Isoelectric Point: 4.4607

>AT204443 WP_004852307.1 NZ_CP075881:3054687-3054944 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT204443 NZ_CP100728:3547961-3548335 [Salmonella enterica subsp. enterica serovar Blockley]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3HDW8