204440

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1143953..1144500 Replicon chromosome
Accession NZ_CP075881
Organism Klebsiella michiganensis strain JNQH491

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag KLH67_RS05285 Protein ID WP_014227299.1
Coordinates 1143953..1144261 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag KLH67_RS05290 Protein ID WP_014227298.1
Coordinates 1144264..1144500 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KLH67_RS05260 (KLH67_05260) 1139857..1140168 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
KLH67_RS05265 (KLH67_05265) 1140463..1141404 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
KLH67_RS05270 (KLH67_05270) 1141428..1141721 - 294 WP_049083313.1 YicS family protein -
KLH67_RS05275 (KLH67_05275) 1141931..1142896 + 966 WP_049083312.1 Rpn family recombination-promoting nuclease/putative transposase -
KLH67_RS05280 (KLH67_05280) 1142900..1143802 - 903 WP_014227300.1 EamA family transporter -
KLH67_RS05285 (KLH67_05285) 1143953..1144261 - 309 WP_014227299.1 CcdB family protein Toxin
KLH67_RS05290 (KLH67_05290) 1144264..1144500 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KLH67_RS05295 (KLH67_05295) 1144606..1146039 - 1434 WP_049083310.1 PTS N-acetylmuramic acid transporter subunit IIBC -
KLH67_RS05300 (KLH67_05300) 1146064..1146966 - 903 WP_032752002.1 N-acetylmuramic acid 6-phosphate etherase -
KLH67_RS05305 (KLH67_05305) 1147127..1148293 - 1167 WP_049083308.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T204440 WP_014227299.1 NZ_CP075881:c1144261-1143953 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T204440 NZ_CP100728:c1041860-1041333 [Salmonella enterica subsp. enterica serovar Blockley]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTATGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGCCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT204440 WP_014227298.1 NZ_CP075881:c1144500-1144264 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT204440 NZ_CP100728:c1042147-1041857 [Salmonella enterica subsp. enterica serovar Blockley]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References