Detailed information of TA system TA204438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 340418..340994
• Replicon: chromosome
• Accession: NZ_CP075881
• Organism: Klebsiella michiganensis strain JNQH491

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1Q8YXR6
• Locus tag: KLH67_RS01545
• Protein ID: WP_046878034.1
• Coordinates: 340707..340994 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: KLH67_RS01540
• Protein ID: WP_014227757.1
• Coordinates: 340418..340720 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KLH67_RS01525 (KLH67_01525)	336478..337458	-	981	WP_032747738.1	IS110 family transposase	-
KLH67_RS01530 (KLH67_01530)	338046..338957	+	912	WP_049082927.1	acetamidase/formamidase family protein	-
KLH67_RS01535 (KLH67_01535)	338954..340297	+	1344	WP_154904524.1	APC family permease	-
KLH67_RS01540 (KLH67_01540)	340418..340720	-	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
KLH67_RS01545 (KLH67_01545)	340707..340994	-	288	WP_046878034.1	BrnT family toxin	Toxin
KLH67_RS01550 (KLH67_01550)	341254..341697	-	444	Protein_307	FosA family fosfomycin resistance glutathione transferase	-
KLH67_RS01555 (KLH67_01555)	341691..342599	-	909	WP_049083710.1	LysR family transcriptional regulator	-
KLH67_RS01560 (KLH67_01560)	342687..343469	+	783	WP_049083709.1	NAD(P)H-dependent oxidoreductase	-
KLH67_RS01565 (KLH67_01565)	343617..344201	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
KLH67_RS01570 (KLH67_01570)	344347..345147	+	801	WP_046878031.1	winged helix-turn-helix domain-containing protein	-
KLH67_RS01575 (KLH67_01575)	345144..345662	+	519	WP_004098240.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IS/Tn	fosA	-	336478..341697	5219

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11210.69 Da
• Isoelectric Point: 7.4687

>T204438 WP_046878034.1 NZ_CP075881:c340994-340707 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T204438 NZ_CP100728:876511-876924 [Salmonella enterica subsp. enterica serovar Blockley]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATATTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT204438 WP_014227757.1 NZ_CP075881:c340720-340418 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT204438 NZ_CP100728:876264-876530 [Salmonella enterica subsp. enterica serovar Blockley]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A1Q8YXR6

Antitoxin

• AlphaFold DB: A0A0H3H591