Detailed information of TA system TA204436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 2162713..2163290
• Replicon: chromosome
• Accession: NZ_CP075872
• Organism: Listeria monocytogenes strain C7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: G8O93_RS10970
• Protein ID: WP_003728465.1
• Coordinates: 2162949..2163290 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: G8O93_RS10965
• Protein ID: WP_003728464.1
• Coordinates: 2162713..2162955 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8O93_RS10930	2158409..2159089	+	681	WP_002389568.1	IS6 family transposase	-
G8O93_RS10935	2159118..2160047	-	930	WP_031672448.1	AAA family ATPase	-
G8O93_RS15705	2160239..2160487	-	249	WP_009911232.1	hypothetical protein	-
G8O93_RS10945	2160886..2161263	-	378	WP_003769263.1	DUF3850 domain-containing protein	-
G8O93_RS10950	2161282..2161944	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
G8O93_RS10955	2161944..2162270	-	327	WP_031641563.1	hypothetical protein	-
G8O93_RS10960	2162416..2162652	+	237	WP_003769265.1	hypothetical protein	-
G8O93_RS10965	2162713..2162955	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G8O93_RS10970	2162949..2163290	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G8O93_RS10975	2163316..2163459	-	144	WP_157105793.1	hypothetical protein	-
G8O93_RS10980	2163513..2164484	-	972	WP_031641941.1	hypothetical protein	-
G8O93_RS10985	2164488..2164742	-	255	WP_031641560.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	clpC	2118896..2238653	119757
	-	flank	IS/Tn	-	-	2158409..2159089	680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.74 Da
• Isoelectric Point: 9.2830

>T204436 WP_003728465.1 NZ_CP075872:2162949-2163290 [Listeria monocytogenes]
MVKQGDIIKINLNPKQGHEQQGYRPYICLSHHLVSDYANIAIFAPISNTPRTYPLYVPLTGTTTTGKVLLDQLVTIDYNA
RKHQYVETVSDPLLTKLLDTVKVIFQKNERSTN

Nucleotide

• Length: 342 bp

>T204436 NZ_CP100728:c350333-349953 [Salmonella enterica subsp. enterica serovar Blockley]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9207.48 Da
• Isoelectric Point: 4.3867

>AT204436 WP_003728464.1 NZ_CP075872:2162713-2162955 [Listeria monocytogenes]
MTTVSVKKWGNSKAVRLPNNLLNALDIKENDDLKVEVINNQMILTKTAIDLRIEDLFANYQGEPFQTELQTFEPIGDEKW

Nucleotide

• Length: 243 bp

>AT204436 NZ_CP100728:c350554-350333 [Salmonella enterica subsp. enterica serovar Blockley]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTACTGAAGTGCTCAACAATGTTGAAGCTGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5