Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2207667..2208164 Replicon chromosome
Accession NZ_CP075852
Organism Escherichia coli strain S19-651

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JFY28_RS10905 Protein ID WP_001173343.1
Coordinates 2207667..2207840 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JFY28_RS10910 Protein ID WP_000398619.1
Coordinates 2207868..2208164 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY28_RS10895 (2204337) 2204337..2205794 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
JFY28_RS10900 (2206031) 2206031..2207548 + 1518 WP_001295074.1 lysine--tRNA ligase -
JFY28_RS10905 (2207667) 2207667..2207840 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JFY28_RS10910 (2207868) 2207868..2208164 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JFY28_RS10915 (2208391) 2208391..2208663 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JFY28_RS10920 (2208675) 2208675..2208905 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
JFY28_RS10925 (2209086) 2209086..2210717 + 1632 WP_001216487.1 sensor histidine kinase -
JFY28_RS10930 (2210714) 2210714..2211433 + 720 WP_032331011.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T204410 WP_001173343.1 NZ_CP075852:c2207840-2207667 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T204410 NZ_CP100718:c4789347-4788973 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAGCGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTAACGCG
TAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACACCGTATCACTTGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT204410 WP_000398619.1 NZ_CP075852:c2208164-2207868 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT204410 NZ_CP100718:c4789589-4789347 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGACGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTGCCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References