Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1463549..1464386 Replicon chromosome
Accession NZ_CP075852
Organism Escherichia coli strain S19-651

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag JFY28_RS07440 Protein ID WP_000227784.1
Coordinates 1463844..1464386 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JFY28_RS07435 Protein ID WP_001297137.1
Coordinates 1463549..1463860 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY28_RS07410 (1458569) 1458569..1459516 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
JFY28_RS07415 (1459538) 1459538..1461529 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JFY28_RS07420 (1461519) 1461519..1462133 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JFY28_RS07425 (1462133) 1462133..1462462 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JFY28_RS07430 (1462474) 1462474..1463364 + 891 WP_000971336.1 heme o synthase -
JFY28_RS07435 (1463549) 1463549..1463860 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JFY28_RS07440 (1463844) 1463844..1464386 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
JFY28_RS07445 (1464442) 1464442..1465377 - 936 WP_001368479.1 tetratricopeptide repeat protein -
JFY28_RS07450 (1465785) 1465785..1467149 + 1365 WP_001000974.1 MFS transporter -
JFY28_RS07455 (1467277) 1467277..1467768 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JFY28_RS07460 (1467936) 1467936..1468847 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T204405 WP_000227784.1 NZ_CP075852:1463844-1464386 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T204405 NZ_CP100718:c4232118-4231831 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT204405 WP_001297137.1 NZ_CP075852:1463549-1463860 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT204405 NZ_CP100718:c4231844-4231542 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGTGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACGCAGGCCTCTGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAAGCGATGCTGCGAGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References