Detailed information of TA system TA204394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1981..2540
• Replicon: plasmid pCPF6-IncI1
• Accession: NZ_CP075740
• Organism: Escherichia coli strain CPF6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: KKZ13_RS24425
• Protein ID: WP_000421259.1
• Coordinates: 2265..2540 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: KKZ13_RS24420
• Protein ID: WP_001178087.1
• Coordinates: 1981..2265 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ13_RS24415 (46)	46..1077	+	1032	WP_052980407.1	plasmid replication initiator RepA	-
KKZ13_RS24420 (1981)	1981..2265	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
KKZ13_RS24425 (2265)	2265..2540	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KKZ13_RS24430 (2646)	2646..2849	+	204	Protein_3	hypothetical protein	-
KKZ13_RS24435 (3351)	3351..6236	-	2886	WP_001138082.1	Tn3 family transposase	-
KKZ13_RS24440 (6362)	6362..6559	+	198	Protein_5	recombinase family protein	-
KKZ13_RS24445 (6613)	6613..7317	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..95375	95375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T204394 WP_000421259.1 NZ_CP075740:2265-2540 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T204394 NZ_CP100715:c4759152-4758841 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT204394 WP_001178087.1 NZ_CP075740:1981-2265 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT204394 NZ_CP100715:c4758840-4758550 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625