Detailed information of TA system TA204393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 41748..42546
• Replicon: plasmid pCPF6-IncFIB
• Accession: NZ_CP075739
• Organism: Escherichia coli strain CPF6

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KKZ13_RS24025
• Protein ID: WP_247852443.1
• Coordinates: 42025..42546 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: KKZ13_RS24020
• Protein ID: WP_001351987.1
• Coordinates: 41748..42017 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ13_RS23995 (KKZ13_23850)	37175..38515	-	1341	WP_000137333.1	DnaB-like helicase C-terminal domain-containing protein	-
KKZ13_RS24000 (KKZ13_23855)	38559..39299	-	741	WP_001717320.1	hypothetical protein	-
KKZ13_RS24005 (KKZ13_23860)	39582..40349	+	768	WP_000342417.1	hypothetical protein	-
KKZ13_RS24010 (KKZ13_23865)	40402..40755	-	354	WP_160394000.1	hypothetical protein	-
KKZ13_RS24015 (KKZ13_23870)	40761..41429	-	669	WP_053902532.1	division plane positioning ATPase MipZ	-
KKZ13_RS24020 (KKZ13_23875)	41748..42017	+	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
KKZ13_RS24025 (KKZ13_23880)	42025..42546	+	522	WP_247852443.1	GNAT family N-acetyltransferase	Toxin
KKZ13_RS24030 (KKZ13_23885)	42715..42966	-	252	WP_000901559.1	hypothetical protein	-
KKZ13_RS24035 (KKZ13_23890)	42968..43660	-	693	WP_032084069.1	membrane protein	-
KKZ13_RS24040 (KKZ13_23895)	43674..43997	-	324	WP_042863551.1	hypothetical protein	-
KKZ13_RS24045 (KKZ13_23900)	44072..44854	-	783	WP_256626239.1	receptor-recognizing protein	-
KKZ13_RS24050	44920..45321	-	402	WP_244461651.1	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..116264	116264

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19420.19 Da
• Isoelectric Point: 8.6394

>T204393 WP_247852443.1 NZ_CP075739:42025-42546 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T204393 NZ_CP100715:c4398012-4397521 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT204393 WP_001351987.1 NZ_CP075739:41748-42017 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT204393 NZ_CP100715:c4398302-4398009 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7