Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 85301..86924 Replicon plasmid unnamed1
Accession NZ_CP075738
Organism Escherichia coli strain CPF6

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KKZ13_RS23020 Protein ID WP_000088046.1
Coordinates 85599..86924 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KKZ13_RS23015 Protein ID WP_024136327.1
Coordinates 85301..85594 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ13_RS22985 (KKZ13_22865) 81173..82414 + 1242 WP_094309018.1 VWA domain-containing protein -
KKZ13_RS22990 82505..82636 + 132 WP_000374059.1 hypothetical protein -
KKZ13_RS22995 (KKZ13_22870) 82977..83258 - 282 WP_000077926.1 hypothetical protein -
KKZ13_RS23000 (KKZ13_22875) 83308..83499 - 192 WP_000398480.1 hypothetical protein -
KKZ13_RS23005 (KKZ13_22880) 83591..83932 - 342 WP_001151575.1 hypothetical protein -
KKZ13_RS23010 (KKZ13_22885) 84305..84697 + 393 WP_000341066.1 hypothetical protein -
KKZ13_RS23015 (KKZ13_22890) 85301..85594 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KKZ13_RS23020 (KKZ13_22895) 85599..86924 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KKZ13_RS23025 (KKZ13_22900) 86985..87191 + 207 WP_000134171.1 hypothetical protein -
KKZ13_RS23030 (KKZ13_22905) 87293..87703 + 411 WP_000985911.1 hypothetical protein -
KKZ13_RS23035 (KKZ13_22910) 87716..88531 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
KKZ13_RS23040 (KKZ13_22915) 88785..89210 + 426 WP_001043843.1 hypothetical protein -
KKZ13_RS23045 (KKZ13_22920) 89759..90067 + 309 WP_001224687.1 hypothetical protein -
KKZ13_RS23050 (KKZ13_22925) 90083..90940 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KKZ13_RS23055 (KKZ13_22930) 91002..91205 + 204 WP_001194555.1 hypothetical protein -
KKZ13_RS23060 (KKZ13_22935) 91547..91903 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-3.2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / tet(A) / sul3 / aac(3)-IId / qnrS1 / blaCTX-M-55 - 1..222848 222848


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T204392 WP_000088046.1 NZ_CP075738:85599-86924 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T204392 NZ_CP100715:c4187727-4187443 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT204392 WP_024136327.1 NZ_CP075738:85301-85594 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT204392 NZ_CP100715:c4187959-4187717 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References