Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4227170..4227788 Replicon chromosome
Accession NZ_CP075737
Organism Escherichia coli strain CPF6

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KKZ13_RS20220 Protein ID WP_001291435.1
Coordinates 4227170..4227388 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KKZ13_RS20225 Protein ID WP_000344800.1
Coordinates 4227414..4227788 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ13_RS20185 (4222459) 4222459..4223031 + 573 WP_000779838.1 YbaY family lipoprotein -
KKZ13_RS20190 (4223062) 4223062..4223373 - 312 WP_000409911.1 MGMT family protein -
KKZ13_RS20200 (4223752) 4223752..4224105 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KKZ13_RS20205 (4224147) 4224147..4225697 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KKZ13_RS20210 (4225861) 4225861..4226331 - 471 WP_000136192.1 YlaC family protein -
KKZ13_RS20215 (4226447) 4226447..4226998 - 552 WP_000102564.1 maltose O-acetyltransferase -
KKZ13_RS20220 (4227170) 4227170..4227388 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KKZ13_RS20225 (4227414) 4227414..4227788 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KKZ13_RS20230 (4228334) 4228334..4231483 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KKZ13_RS20235 (4231506) 4231506..4232699 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204391 WP_001291435.1 NZ_CP075737:c4227388-4227170 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204391 NZ_CP100715:c4093758-4093471 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204391 WP_000344800.1 NZ_CP075737:c4227788-4227414 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204391 NZ_CP100715:c4093484-4093182 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References