Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3701476..3702052 Replicon chromosome
Accession NZ_CP075737
Organism Escherichia coli strain CPF6

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KKZ13_RS17690 Protein ID WP_001297643.1
Coordinates 3701476..3701763 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KKZ13_RS17695 Protein ID WP_000063156.1
Coordinates 3701750..3702052 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ13_RS17670 (3697729) 3697729..3699009 - 1281 WP_001037380.1 DUF445 domain-containing protein -
KKZ13_RS17675 (3699050) 3699050..3700282 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
KKZ13_RS17680 (3700428) 3700428..3700523 - 96 Protein_3445 dienelactone hydrolase family protein -
KKZ13_RS17685 (3700580) 3700580..3701277 + 698 WP_223409538.1 IS1 family transposase -
KKZ13_RS17690 (3701476) 3701476..3701763 + 288 WP_001297643.1 BrnT family toxin Toxin
KKZ13_RS17695 (3701750) 3701750..3702052 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KKZ13_RS17700 (3702086) 3702086..3703042 - 957 WP_001297640.1 GTPase -
KKZ13_RS17705 (3703053) 3703053..3703256 - 204 WP_000467859.1 YbdD/YjiX family protein -
KKZ13_RS17710 (3703306) 3703306..3705456 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3700984..3701277 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T204388 WP_001297643.1 NZ_CP075737:3701476-3701763 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T204388 NZ_CP100715:2425085-2425369 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT204388 WP_000063156.1 NZ_CP075737:3701750-3702052 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT204388 NZ_CP100715:2424847-2425095 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References