Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3494230..3494727 Replicon chromosome
Accession NZ_CP075737
Organism Escherichia coli strain CPF6

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KKZ13_RS16625 Protein ID WP_001173343.1
Coordinates 3494554..3494727 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KKZ13_RS16620 Protein ID WP_000398619.1
Coordinates 3494230..3494526 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ13_RS16600 (3490961) 3490961..3491680 - 720 WP_000611288.1 two-component system response regulator DcuR -
KKZ13_RS16605 (3491677) 3491677..3493308 - 1632 WP_001216477.1 sensor histidine kinase -
KKZ13_RS16610 (3493489) 3493489..3493719 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KKZ13_RS16615 (3493731) 3493731..3494003 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
KKZ13_RS16620 (3494230) 3494230..3494526 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KKZ13_RS16625 (3494554) 3494554..3494727 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KKZ13_RS16630 (3494846) 3494846..3496363 - 1518 WP_001295074.1 lysine--tRNA ligase -
KKZ13_RS16635 (3496600) 3496600..3498057 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T204387 WP_001173343.1 NZ_CP075737:3494554-3494727 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T204387 NZ_CP100715:2083761-2083864 [Salmonella enterica subsp. enterica serovar Typhimurium]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT204387 WP_000398619.1 NZ_CP075737:3494230-3494526 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT204387 NZ_CP100715:c2083866-2083721 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References