Detailed information of TA system TA204383

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2216658..2217456
• Replicon: chromosome
• Accession: NZ_CP075737
• Organism: Escherichia coli strain CPF6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KKZ13_RS10550
• Protein ID: WP_057916008.1
• Coordinates: 2217079..2217456 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L4NTN5
• Locus tag: KKZ13_RS10545
• Protein ID: WP_001605875.1
• Coordinates: 2216658..2217032 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ13_RS10525 (2214389)	2214389..2215207	+	819	WP_001234393.1	DUF932 domain-containing protein	-
KKZ13_RS10530 (2215299)	2215299..2215784	+	486	WP_256626172.1	antirestriction protein	-
KKZ13_RS10535 (2215800)	2215800..2216276	+	477	WP_001186745.1	RadC family protein	-
KKZ13_RS10540 (2216363)	2216363..2216584	+	222	WP_000692350.1	DUF987 domain-containing protein	-
KKZ13_RS10545 (2216658)	2216658..2217032	+	375	WP_001605875.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KKZ13_RS10550 (2217079)	2217079..2217456	+	378	WP_057916008.1	TA system toxin CbtA family protein	Toxin
KKZ13_RS10555 (2217453)	2217453..2217602	+	150	Protein_2066	DUF5983 family protein	-
KKZ13_RS10560 (2217678)	2217678..2217875	+	198	WP_023563519.1	DUF957 domain-containing protein	-
KKZ13_RS10565 (2217960)	2217960..2218802	+	843	WP_001529559.1	DUF4942 domain-containing protein	-
KKZ13_RS10570 (2219083)	2219083..2219619	-	537	WP_024238456.1	GspM family type II secretion system protein YghD	-
KKZ13_RS10575 (2219621)	2219621..2220799	-	1179	WP_024238457.1	type II secretion system protein GspL	-
KKZ13_RS10580 (2220796)	2220796..2221773	-	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
KKZ13_RS10585 (2221776)	2221776..2222375	-	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14140.14 Da
• Isoelectric Point: 7.2652

>T204383 WP_057916008.1 NZ_CP075737:2217079-2217456 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATSLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T204383 NZ_CP100715:365061-365336 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13594.42 Da
• Isoelectric Point: 5.4554

>AT204383 WP_001605875.1 NZ_CP075737:2216658-2217032 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT204383 NZ_CP100715:364798-365058 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1L4NTN5