Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2070935..2071589 Replicon chromosome
Accession NZ_CP075737
Organism Escherichia coli strain CPF6

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KKZ13_RS09885 Protein ID WP_000244777.1
Coordinates 2070935..2071342 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KKZ13_RS09890 Protein ID WP_000354046.1
Coordinates 2071323..2071589 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ13_RS09865 (2066892) 2066892..2068625 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
KKZ13_RS09870 (2068631) 2068631..2069341 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KKZ13_RS09875 (2069366) 2069366..2070262 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KKZ13_RS09880 (2070374) 2070374..2070895 + 522 WP_001055874.1 flavodoxin FldB -
KKZ13_RS09885 (2070935) 2070935..2071342 - 408 WP_000244777.1 protein YgfX Toxin
KKZ13_RS09890 (2071323) 2071323..2071589 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KKZ13_RS09895 (2071832) 2071832..2072812 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KKZ13_RS09900 (2073008) 2073008..2073667 - 660 WP_000250274.1 hemolysin III family protein -
KKZ13_RS09905 (2073831) 2073831..2074142 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KKZ13_RS09910 (2074187) 2074187..2075620 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
KKZ13_RS09915 (2075677) 2075677..2076420 - 744 WP_000951962.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T204382 WP_000244777.1 NZ_CP075737:c2071342-2070935 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204382 NZ_CP100715:316725-317093 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204382 WP_000354046.1 NZ_CP075737:c2071589-2071323 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204382 NZ_CP100715:316507-316728 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References