Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1050..1642 Replicon plasmid pCP52E-IncX4
Accession NZ_CP075733
Organism Escherichia coli strain CP52E

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag KKZ11_RS23845 Protein ID WP_000520549.1
Coordinates 1050..1298 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag KKZ11_RS23850 Protein ID WP_000681613.1
Coordinates 1295..1642 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ11_RS23835 (KKZ11_23725) 5..304 + 300 WP_000650309.1 hypothetical protein -
KKZ11_RS23840 (KKZ11_23730) 395..745 + 351 WP_000854259.1 hypothetical protein -
KKZ11_RS23845 (KKZ11_23735) 1050..1298 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
KKZ11_RS23850 (KKZ11_23740) 1295..1642 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KKZ11_RS23855 (KKZ11_23745) 2003..2506 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
KKZ11_RS23860 (KKZ11_23750) 2510..3739 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
KKZ11_RS23865 (KKZ11_23755) 3816..4109 + 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
KKZ11_RS23870 (KKZ11_23760) 4106..4321 + 216 WP_000760375.1 EexN family lipoprotein -
KKZ11_RS23875 (KKZ11_23765) 4677..5297 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
KKZ11_RS23880 (KKZ11_23770) 5311..5637 + 327 WP_000008647.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33858 33858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T204368 WP_000520549.1 NZ_CP075733:1050-1298 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T204368 NZ_CP100710:876511-876924 [Salmonella enterica subsp. enterica serovar Blockley]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATATTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT204368 WP_000681613.1 NZ_CP075733:1295-1642 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT204368 NZ_CP100710:876264-876530 [Salmonella enterica subsp. enterica serovar Blockley]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References