Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 106901..107502 Replicon plasmid pCP52E-IncFIB
Accession NZ_CP075732
Organism Escherichia coli strain CP52E

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag KKZ11_RS23775 Protein ID WP_001216034.1
Coordinates 107122..107502 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KKZ11_RS23770 Protein ID WP_001190712.1
Coordinates 106901..107122 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ11_RS23745 (102245) 102245..102949 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KKZ11_RS23750 (102895) 102895..104007 - 1113 WP_024166810.1 class 1 integron integrase IntI1 -
KKZ11_RS23755 (104163) 104163..104636 + 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
KKZ11_RS23760 (104883) 104883..105587 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KKZ11_RS23765 (105642) 105642..106718 - 1077 Protein_116 type I restriction-modification system subunit M -
KKZ11_RS23770 (106901) 106901..107122 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KKZ11_RS23775 (107122) 107122..107502 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KKZ11_RS23780 (107507) 107507..107686 + 180 WP_001513661.1 hypothetical protein -
KKZ11_RS23785 (107714) 107714..107992 + 279 Protein_120 pdcB -
KKZ11_RS23790 (107997) 107997..108410 + 414 Protein_121 integrase core domain-containing protein -
KKZ11_RS23795 (108360) 108360..108695 - 336 WP_169329198.1 type I deoxyribonuclease HsdR -
KKZ11_RS23800 (108905) 108905..109885 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
KKZ11_RS23805 (110129) 110129..111532 + 1404 WP_001373486.1 S-methylmethionine permease -
KKZ11_RS23810 (111519) 111519..112451 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / dfrA14 vat 1..115794 115794
- inside IScluster/Tn dfrA14 - 104163..109885 5722


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T204367 WP_001216034.1 NZ_CP075732:107122-107502 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T204367 NZ_CP100710:c796491-795562 [Salmonella enterica subsp. enterica serovar Blockley]
ATGGATCTGCGTGATAGTTATGCCCGACAGGTCAGGCTATTAATGGCTGCCCTGCCGCATGTGGCAAAGGAGTCCTGTTT
CGCGCTGAAAGGCGGCACGGCAATCAATTTGTTTGTGCAGGATTTCCCTCGCCTGTCTGTAGATATCGATCTGGCGTATA
AGACCTATGCGGATCGCGATACCGATCTGGCTGCCATCAATGACGCCTTGATGCGTATTACTGAATCGCTGAACGGCAGA
CCGGGGATCACTGCGATCCGGCAGGAGAATAAAGCGGATGAAAAGCGCATCATCGTTAACACCGCCGACGCGAAGATAAA
GATTGAGGTTAGTCCGGTCTGGCGTGGGCTGCTGTTGCCTCCGGCGGAAATGCCGGTATGTGAACAGGTAGAAATGGAAT
ATGGCTTCACTACCATGAATGTCGTCTCGCTGGCGGATCTTTACGGCGGTAAAATCTGCGCCGCGCTTGACCGCCAACAT
CCGCGCGATCTGTTTGACGTGCTGAATATGCTGGAGAAACCGGGCCTGACGCGCGAGATCTTTGACGGTTTTCTTTGCTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAACTGCTGGCACCAAACTGGGATACAGCGCGGATCGCTAACCTGTACGCAC
AGGAATTTTCAGGCATGACACAGCAGGAAACATCGCTGGAATCCCTTTTATCAGTAACGACTTTGCTGCCACAAGCGCTG
AAATCGCATCTTACGGACAGGGATCGCCAGCTCTTGCTCAGCTATAAGCAAAATACCCCCGACTGGTCGCTGTATCGCTA
TCCTGAAATTCAACACCTACCGGCGATTCGCTGGAAGCTGCGTAACCTGGCAGTGCTGAATGATAAGAATCCGGCCAAGT
ATGCAGCGGCGGTGAATAAGCTGGAGGGGGTTCTGGAGCAGCATTTTTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT204367 WP_001190712.1 NZ_CP075732:106901-107122 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT204367 NZ_CP100710:c797260-796478 [Salmonella enterica subsp. enterica serovar Blockley]
TTGCCAAACTATCTAAACTGGCTGATGCAGAACACCCTACCCGGTCAGGTGTTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTTTCTTACCTCTATGTGCAGAATGGCTGGCTCAGGCGGATCGCGCACGGCGTATACTGCCGGACGG
GCCGTGAGCCGGACTGGGTGGACGCGGTTTACTGCCTGCAAACGCAGTGGGAAAAACCGGTTCGCGTGGCGGGGCTGACC
AGCCTTGCGCTACAAGGCCACGCTCACTATACCGAACCGGGCAAACAACAAATCTGGCTCGCACTCTCGGCTTACGTCAA
ACTGCCGGGATGGTTTGTCGCCTTTGAACAATTCGCGACGTTTGTCACGCTGTATACCTCTGGCCTCACCGTCGATATAA
CACCATTCACAACAAAACTGAATATCGGCGACCGGCAGTTAGTAGGCAGTGTCCCGGAACTGGCCGCGTATGAGATCGCT
GGCGGCGTTCCTGCGTTGATCTCGTTCGAACATGCCGACGCCCTGTTTCAGGGAATGAACCTTCTCAGCCCGCGTAAATT
ACAGGCCCTGCTCTGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTCTATCTTGCCCGACGCAATGCGCATTCTT
ACTTTCAGTACCTCGAGGAAAGCAGTATAAAAACAGGCTTGGGAAAGCGGCAAATCGTTCCCGGCGGCTGGCTGGAGCCG
GATTATCAAATCACCGTTCCCCGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References