Detailed information of TA system TA204351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3534201..3535038
• Replicon: chromosome
• Accession: NZ_CP075731
• Organism: Escherichia coli strain CP52E

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KKZ11_RS17055
• Protein ID: WP_000227784.1
• Coordinates: 3534496..3535038 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KKZ11_RS17050
• Protein ID: WP_001297137.1
• Coordinates: 3534201..3534512 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ11_RS17025 (3529221)	3529221..3530168	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KKZ11_RS17030 (3530190)	3530190..3532181	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KKZ11_RS17035 (3532171)	3532171..3532785	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KKZ11_RS17040 (3532785)	3532785..3533114	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KKZ11_RS17045 (3533126)	3533126..3534016	+	891	WP_000971336.1	heme o synthase	-
KKZ11_RS17050 (3534201)	3534201..3534512	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KKZ11_RS17055 (3534496)	3534496..3535038	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KKZ11_RS17060 (3535094)	3535094..3536029	-	936	WP_136698416.1	tetratricopeptide repeat protein	-
KKZ11_RS17065 (3536437)	3536437..3537801	+	1365	WP_001000954.1	MFS transporter	-
KKZ11_RS17070 (3537929)	3537929..3538420	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KKZ11_RS17075 (3538588)	3538588..3539499	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T204351 WP_000227784.1 NZ_CP075731:3534496-3535038 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T204351 NZ_CP100707:881459-881857 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT204351 WP_001297137.1 NZ_CP075731:3534201-3534512 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT204351 NZ_CP100707:881232-881459 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3