Detailed information of TA system TA204349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2441014..2441652
• Replicon: chromosome
• Accession: NZ_CP075731
• Organism: Escherichia coli strain CP52E

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2X7GI71
• Locus tag: KKZ11_RS11680
• Protein ID: WP_032231106.1
• Coordinates: 2441476..2441652 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KKZ11_RS11675
• Protein ID: WP_001270286.1
• Coordinates: 2441014..2441430 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ11_RS11655 (2436166)	2436166..2437107	-	942	WP_001251331.1	ABC transporter permease	-
KKZ11_RS11660 (2437108)	2437108..2438121	-	1014	WP_136698316.1	ABC transporter ATP-binding protein	-
KKZ11_RS11665 (2438139)	2438139..2439284	-	1146	WP_000047425.1	ABC transporter substrate-binding protein	-
KKZ11_RS11670 (2439529)	2439529..2440935	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KKZ11_RS11675 (2441014)	2441014..2441430	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KKZ11_RS11680 (2441476)	2441476..2441652	-	177	WP_032231106.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KKZ11_RS11685 (2441874)	2441874..2442104	+	231	WP_000494244.1	YncJ family protein	-
KKZ11_RS11690 (2442196)	2442196..2444157	-	1962	WP_136698317.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KKZ11_RS11695 (2444230)	2444230..2444766	-	537	WP_000429151.1	DNA-binding transcriptional regulator SutR	-
KKZ11_RS11700 (2444858)	2444858..2445894	+	1037	Protein_2290	benzoate/H(+) symporter BenE family transporter	-
KKZ11_RS11705 (2445929)	2445929..2446597	-	669	WP_001261021.1	DUF3313 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6680.72 Da
• Isoelectric Point: 10.9223

>T204349 WP_032231106.1 NZ_CP075731:c2441652-2441476 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPCHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204349 NZ_CP100707:365061-365336 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT204349 WP_001270286.1 NZ_CP075731:c2441430-2441014 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204349 NZ_CP100707:364798-365058 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A2X7GI71