Detailed information of TA system TA204338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1019966..1020549
• Replicon: chromosome
• Accession: NZ_CP075731
• Organism: Escherichia coli strain CP52E

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KKZ11_RS04950
• Protein ID: WP_089601808.1
• Coordinates: 1020214..1020549 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A161R357
• Locus tag: KKZ11_RS04945
• Protein ID: WP_040062758.1
• Coordinates: 1019966..1020214 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ11_RS04935 (1016305)	1016305..1017606	+	1302	WP_136698165.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
KKZ11_RS04940 (1017654)	1017654..1019888	+	2235	WP_000226803.1	GTP diphosphokinase	-
KKZ11_RS04945 (1019966)	1019966..1020214	+	249	WP_040062758.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KKZ11_RS04950 (1020214)	1020214..1020549	+	336	WP_089601808.1	endoribonuclease MazF	Toxin
KKZ11_RS04955 (1020620)	1020620..1021411	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
KKZ11_RS04960 (1021639)	1021639..1023276	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
KKZ11_RS04965 (1023364)	1023364..1024662	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
KKZ11_RS04970 (1024806)	1024806..1024999	+	194	Protein_974	transposase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• Antitoxin PemI-like (2-82)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12142.09 Da
• Isoelectric Point: 8.2618

>T204338 WP_089601808.1 NZ_CP075731:1020214-1020549 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKSSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTLAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T204338 NZ_CP100702:2318202-2318486 [Salmonella enterica subsp. enterica serovar Thompson]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9297.70 Da
• Isoelectric Point: 4.5972

>AT204338 WP_040062758.1 NZ_CP075731:1019966-1020214 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNGITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT204338 NZ_CP100702:2317964-2318212 [Salmonella enterica subsp. enterica serovar Thompson]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A161R357