Detailed information of TA system TA204333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 57987..58509
• Replicon: plasmid unnamed.1
• Accession: NZ_CP075724
• Organism: Escherichia coli strain CPE35

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: KKZ10_RS23310
• Protein ID: WP_000220560.1
• Coordinates: 57987..58268 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: KKZ10_RS23315
• Protein ID: WP_000121743.1
• Coordinates: 58258..58509 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ10_RS23285 (KKZ10_23175)	53186..53419	-	234	WP_000624775.1	hypothetical protein	-
KKZ10_RS23290 (KKZ10_23180)	53444..54148	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KKZ10_RS23295 (KKZ10_23185)	54202..56511	+	2310	Protein_65	Tn3 family transposase	-
KKZ10_RS23300 (KKZ10_23190)	56718..57221	+	504	WP_241088267.1	restriction endonuclease	-
KKZ10_RS23310 (KKZ10_23200)	57987..58268	-	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KKZ10_RS23315 (KKZ10_23205)	58258..58509	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
KKZ10_RS23320 (KKZ10_23210)	59074..59262	+	189	WP_048230572.1	hypothetical protein	-
KKZ10_RS23325 (KKZ10_23215)	59272..60471	+	1200	WP_094316173.1	IS91 family transposase	-
KKZ10_RS23330 (KKZ10_23220)	61453..62289	+	837	WP_001050931.1	RepB family plasmid replication initiator protein	-
KKZ10_RS23335 (KKZ10_23225)	62329..62775	+	447	Protein_73	hypothetical protein	-
KKZ10_RS23340 (KKZ10_23230)	62912..63268	+	357	WP_000456533.1	DNA distortion polypeptide 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(3'')-Ia / blaTEM-1B / tet(M) / aph(3')-Ia / sul3 / cmlA1 / aadA2 / dfrA12 / aph(3'')-Ib / aph(6)-Id	-	1..91697	91697
	-	inside	IScluster/Tn	ant(3'')-Ia / blaTEM-1B / tet(M) / aph(3')-Ia / sul3 / cmlA1 / aadA2 / dfrA12 / aph(3'')-Ib / aph(6)-Id	-	31768..90786	59018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T204333 WP_000220560.1 NZ_CP075724:c58268-57987 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T204333 NZ_CP100702:315437-315805 [Salmonella enterica subsp. enterica serovar Thompson]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTTGCTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT204333 WP_000121743.1 NZ_CP075724:c58509-58258 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT204333 NZ_CP100702:315219-315440 [Salmonella enterica subsp. enterica serovar Thompson]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301