Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3395592..3396210 Replicon chromosome
Accession NZ_CP075722
Organism Escherichia coli strain CPE35

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KKZ10_RS16440 Protein ID WP_001291435.1
Coordinates 3395992..3396210 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KKZ10_RS16435 Protein ID WP_000344800.1
Coordinates 3395592..3395966 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ10_RS16425 (3390681) 3390681..3391874 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KKZ10_RS16430 (3391897) 3391897..3395046 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KKZ10_RS16435 (3395592) 3395592..3395966 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KKZ10_RS16440 (3395992) 3395992..3396210 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KKZ10_RS16445 (3396382) 3396382..3396933 + 552 WP_000102564.1 maltose O-acetyltransferase -
KKZ10_RS16450 (3397049) 3397049..3397519 + 471 WP_000136192.1 YlaC family protein -
KKZ10_RS16455 (3397683) 3397683..3399233 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KKZ10_RS16460 (3399275) 3399275..3399628 - 354 WP_256633942.1 DUF1428 family protein -
KKZ10_RS16470 (3400007) 3400007..3400318 + 312 WP_000409911.1 MGMT family protein -
KKZ10_RS16475 (3400349) 3400349..3400921 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204327 WP_001291435.1 NZ_CP075722:3395992-3396210 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204327 NZ_CP100698:4111861-4112277 [Salmonella enterica subsp. enterica serovar Agona]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCGGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204327 WP_000344800.1 NZ_CP075722:3395592-3395966 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204327 NZ_CP100698:4111634-4111864 [Salmonella enterica subsp. enterica serovar Agona]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References