Detailed information of TA system TA204314

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1115802..1116469
• Replicon: chromosome
• Accession: NZ_CP075722
• Organism: Escherichia coli strain CPE35

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A156PNF2
• Locus tag: KKZ10_RS05420
• Protein ID: WP_001618791.1
• Coordinates: 1115802..1116131 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: KKZ10_RS05425
• Protein ID: WP_256633971.1
• Coordinates: 1116152..1116469 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ10_RS05415 (1110857)	1110857..1115437	+	4581	WP_001581967.1	adhesin-like autotransporter YpjA/EhaD	-
KKZ10_RS05420 (1115802)	1115802..1116131	-	330	WP_001618791.1	TA system toxin CbtA family protein	Toxin
KKZ10_RS05425 (1116152)	1116152..1116469	-	318	WP_256633971.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KKZ10_RS05430 (1116488)	1116488..1116709	-	222	WP_063928002.1	DUF987 domain-containing protein	-
KKZ10_RS05435 (1116718)	1116718..1117194	-	477	WP_000811693.1	RadC family protein	-
KKZ10_RS05440 (1117210)	1117210..1117668	-	459	WP_256633973.1	antirestriction protein	-
KKZ10_RS05445 (1117770)	1117770..1118009	-	240	WP_000194654.1	DUF905 family protein	-
KKZ10_RS05450 (1118086)	1118086..1118553	-	468	WP_256633974.1	protein YkfB	-
KKZ10_RS05455 (1118577)	1118577..1119020	-	444	WP_000824224.1	hypothetical protein	-
KKZ10_RS05460 (1119020)	1119020..1119256	-	237	WP_001115854.1	protein YpjK	-
KKZ10_RS05465 (1119293)	1119293..1119994	-	702	WP_001548933.1	WYL domain-containing protein	-
KKZ10_RS05470 (1120211)	1120211..1121032	-	822	WP_021574424.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1106149..1153711	47562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12509.43 Da
• Isoelectric Point: 7.2767

>T204314 WP_001618791.1 NZ_CP075722:c1116131-1115802 [Escherichia coli]
MNTLPATTQRAAKPCLSPVAVWKMLLTHLLEQHYGLTLNDTPFSEERVIQEHIDAGITLADTVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTDLLKTNVK

Nucleotide

• Length: 330 bp

>T204314 NZ_CP100696:13961-14443 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGGAAATAAGTGTTACTGCACCGGAGTTACTAAACGAGGAACACGATCTTCAACAGTTTGATTGCGGGAATGATGTGCT
AAGTGACTGGCTTCGCCGCAGGGCGATGAAGAACCAATACCTCAATGCTTCCCGCACATTTGTTATCTGCCCTGAATGCA
CAAAGCGAGTAGTGGGATATTATTCTATTGCGACGGGGTCCGTAAGCCATGACAGTTTAGGTAGAAGTTTACGCCAAAAT
ATGCCCGATCCAGTTCCGGTTGTTTTGCTGGGGCGTCTTGCTGTTGATGAGTGTACTCAAGGGCATAGTTTTGGAAAATG
GCTGCTTAATGATACTGTAACACGAATATCTAATCTTGCAGACCAGGTGGGTATTAAAGCGATCATGGTTCATGCTATTG
ATGAACAGGCGAAAACATTCTATGAATACTTTGGCTTTGTTCAGTCTCCCATTTCGCCAAACATATTGTTCTACAAGATT
TAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11992.57 Da
• Isoelectric Point: 6.9466

>AT204314 WP_256633971.1 NZ_CP075722:c1116469-1116152 [Escherichia coli]
MSNITWGLQRAITPRQEARLVQEGNRLHYLADRASITGRFSDAECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT204314 NZ_CP100696:13686-13970 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGCAAACTACAATCCGCAAATCTGTGCGTAATAACCAAATAAATATACGGGCTACTGATGAGGAGCGTGCTGTGATTGA
TTATGCAGCAAGTCTGGTCAGTAAAAATCGTACCGATTTCATCATAGAAAAAGCTGTGAGTGAGGCCCAGAATATCATTC
TGGATCAGCGTGTTTTCGTTTTGGATGATGCCCGCTATCAGGCTTTTATTAAACAGCTTGAAGCCCCTGTACAGAATACT
GAAGGTCGTCAGCGCTTGATGGATGTGAAACCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A156PNF2