Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 843277..843931 Replicon chromosome
Accession NZ_CP075722
Organism Escherichia coli strain CPE35

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KKZ10_RS04140 Protein ID WP_000244777.1
Coordinates 843524..843931 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KKZ10_RS04135 Protein ID WP_000354046.1
Coordinates 843277..843543 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ10_RS04110 (838446) 838446..839189 + 744 WP_000951964.1 SDR family oxidoreductase -
KKZ10_RS04115 (839246) 839246..840679 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KKZ10_RS04120 (840724) 840724..841035 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KKZ10_RS04125 (841199) 841199..841858 + 660 WP_000250274.1 hemolysin III family protein -
KKZ10_RS04130 (842054) 842054..843034 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KKZ10_RS04135 (843277) 843277..843543 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KKZ10_RS04140 (843524) 843524..843931 + 408 WP_000244777.1 protein YgfX Toxin
KKZ10_RS04145 (843971) 843971..844492 - 522 WP_001055874.1 flavodoxin FldB -
KKZ10_RS04150 (844604) 844604..845500 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KKZ10_RS04155 (845525) 845525..846235 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KKZ10_RS04160 (846241) 846241..847974 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T204312 WP_000244777.1 NZ_CP075722:843524-843931 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204312 NZ_CP100695:c4750142-4749768 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAGCGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTAACGCG
TAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACACCGTATCACTTGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204312 WP_000354046.1 NZ_CP075722:843277-843543 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204312 NZ_CP100695:c4750384-4750142 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGACGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTGCCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References