Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 150424..150949 Replicon plasmid pCPWW7-IncF
Accession NZ_CP075717
Organism Escherichia coli strain CPww7

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KKZ15_RS24545 Protein ID WP_001159871.1
Coordinates 150424..150729 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag KKZ15_RS24550 Protein ID WP_000813639.1
Coordinates 150731..150949 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ15_RS24520 (145426) 145426..146397 - 972 WP_032248840.1 ParB/RepB/Spo0J family plasmid partition protein -
KKZ15_RS24525 (146397) 146397..147563 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
KKZ15_RS24530 (148151) 148151..148324 - 174 Protein_163 RepB family plasmid replication initiator protein -
KKZ15_RS24540 (149759) 149759..150423 - 665 Protein_165 site-specific integrase -
KKZ15_RS24545 (150424) 150424..150729 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KKZ15_RS24550 (150731) 150731..150949 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KKZ15_RS24555 (151766) 151766..152056 - 291 WP_032248853.1 hypothetical protein -
KKZ15_RS24560 (152053) 152053..153180 - 1128 WP_032248854.1 DUF4238 domain-containing protein -
KKZ15_RS24565 (153214) 153214..154737 - 1524 WP_227464009.1 hypothetical protein -
KKZ15_RS24570 (155014) 155014..155490 - 477 WP_032248785.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..162438 162438


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T204304 WP_001159871.1 NZ_CP075717:c150729-150424 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T204304 NZ_CP100695:c2745805-2745702 [Salmonella enterica subsp. enterica serovar Weltevreden]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT204304 WP_000813639.1 NZ_CP075717:c150949-150731 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT204304 NZ_CP100695:2745700-2745843 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References