Detailed information of TA system TA204300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 101289..101914
• Replicon: plasmid pCPWW7-IncF
• Accession: NZ_CP075717
• Organism: Escherichia coli strain CPww7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KKZ15_RS24230
• Protein ID: WP_000911313.1
• Coordinates: 101516..101914 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: KKZ15_RS24225
• Protein ID: WP_000450532.1
• Coordinates: 101289..101516 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ15_RS24225 (101289)	101289..101516	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KKZ15_RS24230 (101516)	101516..101914	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
KKZ15_RS24235 (101923)	101923..104076	-	2154	WP_072664524.1	type IV conjugative transfer system coupling protein TraD	-
KKZ15_RS24240 (104329)	104329..105039	-	711	WP_230133085.1	conjugal transfer complement resistance protein TraT	-
KKZ15_RS24245 (105092)	105092..105589	-	498	WP_000605859.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..162438	162438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T204300 WP_000911313.1 NZ_CP075717:101516-101914 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T204300 NZ_CP100695:300205-300573 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGGCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT204300 WP_000450532.1 NZ_CP075717:101289-101516 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT204300 NZ_CP100695:299987-300208 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5