Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4406132..4406757 Replicon chromosome
Accession NZ_CP075716
Organism Escherichia coli strain CPww7

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KKZ15_RS21450 Protein ID WP_000911329.1
Coordinates 4406359..4406757 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KKZ15_RS21445 Protein ID WP_000450524.1
Coordinates 4406132..4406359 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ15_RS21420 (4401934) 4401934..4402404 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
KKZ15_RS21425 (4402404) 4402404..4402976 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KKZ15_RS21430 (4403122) 4403122..4404000 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
KKZ15_RS21435 (4404017) 4404017..4405051 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KKZ15_RS21440 (4405264) 4405264..4405977 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KKZ15_RS21445 (4406132) 4406132..4406359 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KKZ15_RS21450 (4406359) 4406359..4406757 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
KKZ15_RS21455 (4406904) 4406904..4407767 + 864 WP_112021547.1 neutral zinc metallopeptidase -
KKZ15_RS21460 (4407782) 4407782..4409797 + 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
KKZ15_RS21465 (4409871) 4409871..4410569 + 699 WP_000679812.1 esterase -
KKZ15_RS21470 (4410679) 4410679..4410879 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T204296 WP_000911329.1 NZ_CP075716:4406359-4406757 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T204296 NZ_CP100693:c4111633-4111349 [Salmonella enterica subsp. enterica serovar London]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT204296 WP_000450524.1 NZ_CP075716:4406132-4406359 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT204296 NZ_CP100693:c4111865-4111623 [Salmonella enterica subsp. enterica serovar London]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References