Detailed information of TA system TA204294

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3976909..3977563
• Replicon: chromosome
• Accession: NZ_CP075716
• Organism: Escherichia coli strain CPww7

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: KKZ15_RS19435
• Protein ID: WP_000244781.1
• Coordinates: 3977156..3977563 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: KKZ15_RS19430
• Protein ID: WP_139501106.1
• Coordinates: 3976909..3977175 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ15_RS19410 (3972997)	3972997..3974430	-	1434	WP_139501107.1	6-phospho-beta-glucosidase BglA	-
KKZ15_RS19415 (3974475)	3974475..3974786	+	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
KKZ15_RS19420 (3974950)	3974950..3975609	+	660	WP_000250274.1	hemolysin III family protein	-
KKZ15_RS19425 (3975686)	3975686..3976666	-	981	WP_000886080.1	tRNA-modifying protein YgfZ	-
KKZ15_RS19430 (3976909)	3976909..3977175	+	267	WP_139501106.1	FAD assembly factor SdhE	Antitoxin
KKZ15_RS19435 (3977156)	3977156..3977563	+	408	WP_000244781.1	protein YgfX	Toxin
KKZ15_RS19440 (3977603)	3977603..3978124	-	522	WP_001055874.1	flavodoxin FldB	-
KKZ15_RS19445 (3978236)	3978236..3979132	+	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
KKZ15_RS19450 (3979157)	3979157..3979867	+	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KKZ15_RS19455 (3979873)	3979873..3981606	+	1734	WP_096262898.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T204294 WP_000244781.1 NZ_CP075716:3977156-3977563 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T204294 NZ_CP100693:3612707-3612982 [Salmonella enterica subsp. enterica serovar London]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGCGACTGGCTGGCAGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGAGCTCCGAGGGGTGTT
TGACTCTGATTGCGGAGCGCGACGAGGTGCAGGAGCTGCGGGAGGAACTTTATCAGGTTAAACAGGTGGTTAAAGGAATG
AAGGTCGGGATATCCGGCGTATTACGCGAGGTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10494.07 Da
• Isoelectric Point: 4.8726

>AT204294 WP_139501106.1 NZ_CP075716:3976909-3977175 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFICLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT204294 NZ_CP100693:c3612931-3612550 [Salmonella enterica subsp. enterica serovar London]
CTGTTTAACCTGATAAAGTTCCTCCCGCAGCTCCTGCACCTCGTCGCGCTCCGCAATCAGAGTCAAACACCCCTCGGAGC
TCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTTCTGCCAGCCAGTCGCCCTTAAGGTGCTGGCTGGGGATTTGT
GAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAAT
AAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAGCGAGTATGGGCGCGACATCCTGGCACCGC
GCCTCCCTCTCCCCGGCCAGTACCGGGCGGTGATGAGCAACCGGCTACCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: S1PAM6