Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3404943..3405743 Replicon chromosome
Accession NZ_CP075716
Organism Escherichia coli strain CPww7

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KKZ15_RS16575 Protein ID WP_000342449.1
Coordinates 3405216..3405743 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KKZ15_RS16570 Protein ID WP_001277108.1
Coordinates 3404943..3405209 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ15_RS16550 (3400601) 3400601..3401269 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KKZ15_RS16555 (3401262) 3401262..3402320 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KKZ15_RS16560 (3402565) 3402565..3403419 + 855 WP_000130221.1 RNA polymerase sigma factor RpoH -
KKZ15_RS16565 (3403690) 3403690..3404793 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KKZ15_RS16570 (3404943) 3404943..3405209 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KKZ15_RS16575 (3405216) 3405216..3405743 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KKZ15_RS16580 (3405740) 3405740..3406123 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
KKZ15_RS16585 (3406547) 3406547..3407656 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KKZ15_RS16590 (3407704) 3407704..3408630 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KKZ15_RS16595 (3408627) 3408627..3409904 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
KKZ15_RS16600 (3409901) 3409901..3410668 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T204292 WP_000342449.1 NZ_CP075716:3405216-3405743 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T204292 NZ_CP100693:2329417-2329701 [Salmonella enterica subsp. enterica serovar London]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT204292 WP_001277108.1 NZ_CP075716:3404943-3405209 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT204292 NZ_CP100693:2329179-2329427 [Salmonella enterica subsp. enterica serovar London]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References