Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3377870..3378501 Replicon chromosome
Accession NZ_CP075716
Organism Escherichia coli strain CPww7

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag KKZ15_RS16415 Protein ID WP_001259385.1
Coordinates 3377870..3378145 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag KKZ15_RS16420 Protein ID WP_000593554.1
Coordinates 3378142..3378501 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ15_RS16400 (3372874) 3372874..3373941 + 1068 WP_000361467.1 HlyD family secretion protein -
KKZ15_RS16405 (3373938) 3373938..3376673 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
KKZ15_RS16410 (3376673) 3376673..3377797 + 1125 WP_001314210.1 ABC transporter permease -
KKZ15_RS16415 (3377870) 3377870..3378145 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
KKZ15_RS16420 (3378142) 3378142..3378501 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KKZ15_RS16425 (3378551) 3378551..3379411 - 861 WP_001131782.1 class II aldolase -
KKZ15_RS16430 (3379443) 3379443..3379712 - 270 WP_000933060.1 HPr family phosphocarrier protein -
KKZ15_RS16435 (3379702) 3379702..3381210 - 1509 WP_001298718.1 FGGY-family carbohydrate kinase -
KKZ15_RS16440 (3381203) 3381203..3382561 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
KKZ15_RS16445 (3382638) 3382638..3382919 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
KKZ15_RS16450 (3382916) 3382916..3383389 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3370253..3384760 14507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T204291 WP_001259385.1 NZ_CP075716:3377870-3378145 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204291 NZ_CP100693:1985479-1985581 [Salmonella enterica subsp. enterica serovar London]
GCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT204291 WP_000593554.1 NZ_CP075716:3378142-3378501 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204291 NZ_CP100693:c1985583-1985440 [Salmonella enterica subsp. enterica serovar London]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References