Detailed information of TA system TA020429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238964..1239532
• Replicon: chromosome
• Accession: NC_002755
• Organism: Mycobacterium tuberculosis CDC1551

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MT_RS05900
• Protein ID: WP_003405865.1
• Coordinates: 1239158..1239532 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MT_RS05895
• Protein ID: WP_003405863.1
• Coordinates: 1238964..1239161 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT_RS05875	1235005..1235643	-	639	WP_003898730.1	lipid droplet-associated protein	-
MT_RS05880	1235733..1236740	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MT_RS05885	1236757..1237740	-	984	WP_003917421.1	hypothetical protein	-
MT_RS05890	1237803..1238876	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
MT_RS05895	1238964..1239161	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MT_RS05900	1239158..1239532	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MT_RS05905	1239735..1240433	+	699	WP_003898733.1	hypothetical protein	-
MT_RS05910	1240551..1240736	+	186	WP_003901093.1	hypothetical protein	-
MT_RS05915	1240663..1240938	-	276	WP_003405867.1	hypothetical protein	-
MT_RS05920	1241181..1241504	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
MT_RS05925	1241519..1242229	-	711	WP_003911399.1	hypothetical protein	-
MT_RS22535	1242251..1242460	+	210	WP_003911400.1	hypothetical protein	-
MT_RS05935	1242412..1243119	-	708	Protein_1161	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T20429 WP_003405865.1 NC_002755:1239158-1239532 [Mycobacterium tuberculosis CDC1551]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T20429 NC_002755:1239158-1239532 [Mycobacterium tuberculosis CDC1551]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT20429 WP_003405863.1 NC_002755:1238964-1239161 [Mycobacterium tuberculosis CDC1551]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT20429 NC_002755:1238964-1239161 [Mycobacterium tuberculosis CDC1551]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07