Detailed information of TA system TA204286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1846817..1847654
• Replicon: chromosome
• Accession: NZ_CP075716
• Organism: Escherichia coli strain CPww7

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: KKZ15_RS09175
• Protein ID: WP_096262872.1
• Coordinates: 1847112..1847654 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KKZ15_RS09170
• Protein ID: WP_001297137.1
• Coordinates: 1846817..1847128 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ15_RS09145 (1841837)	1841837..1842784	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KKZ15_RS09150 (1842806)	1842806..1844797	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KKZ15_RS09155 (1844787)	1844787..1845401	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KKZ15_RS09160 (1845401)	1845401..1845730	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KKZ15_RS09165 (1845742)	1845742..1846632	+	891	WP_000971336.1	heme o synthase	-
KKZ15_RS09170 (1846817)	1846817..1847128	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KKZ15_RS09175 (1847112)	1847112..1847654	+	543	WP_096262872.1	GNAT family N-acetyltransferase	Toxin
KKZ15_RS09180 (1847710)	1847710..1848645	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KKZ15_RS09185 (1849053)	1849053..1850417	+	1365	WP_001000978.1	MFS transporter	-
KKZ15_RS09190 (1850545)	1850545..1851036	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KKZ15_RS09195 (1851204)	1851204..1852115	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19804.03 Da
• Isoelectric Point: 8.6178

>T204286 WP_096262872.1 NZ_CP075716:1847112-1847654 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSNITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T204286 NZ_CP100691:4781787-4782098 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT204286 WP_001297137.1 NZ_CP075716:1846817-1847128 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT204286 NZ_CP100691:4782095-4782541 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3