Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1812739..1813357 Replicon chromosome
Accession NZ_CP075716
Organism Escherichia coli strain CPww7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KKZ15_RS09005 Protein ID WP_001291435.1
Coordinates 1813139..1813357 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KKZ15_RS09000 Protein ID WP_000344800.1
Coordinates 1812739..1813113 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ15_RS08990 (1807828) 1807828..1809021 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KKZ15_RS08995 (1809044) 1809044..1812193 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KKZ15_RS09000 (1812739) 1812739..1813113 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KKZ15_RS09005 (1813139) 1813139..1813357 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KKZ15_RS09010 (1813529) 1813529..1814080 + 552 WP_000102564.1 maltose O-acetyltransferase -
KKZ15_RS09015 (1814196) 1814196..1814666 + 471 WP_000136192.1 YlaC family protein -
KKZ15_RS09020 (1814830) 1814830..1816380 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KKZ15_RS09025 (1816422) 1816422..1816775 - 354 WP_000878141.1 DUF1428 family protein -
KKZ15_RS09035 (1817154) 1817154..1817465 + 312 WP_000409911.1 MGMT family protein -
KKZ15_RS09040 (1817496) 1817496..1818068 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204285 WP_001291435.1 NZ_CP075716:1813139-1813357 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204285 NZ_CP100691:c4780554-4780243 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204285 WP_000344800.1 NZ_CP075716:1812739-1813113 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204285 NZ_CP100691:c4780242-4779952 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References