Detailed information of TA system TA204284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 740048..740686
• Replicon: chromosome
• Accession: NZ_CP075716
• Organism: Escherichia coli strain CPww7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KKZ15_RS03570
• Protein ID: WP_000813794.1
• Coordinates: 740510..740686 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KKZ15_RS03565
• Protein ID: WP_001270286.1
• Coordinates: 740048..740464 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ15_RS03545 (735200)	735200..736141	-	942	WP_001251315.1	ABC transporter permease	-
KKZ15_RS03550 (736142)	736142..737155	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
KKZ15_RS03555 (737173)	737173..738318	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
KKZ15_RS03560 (738563)	738563..739969	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
KKZ15_RS03565 (740048)	740048..740464	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KKZ15_RS03570 (740510)	740510..740686	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KKZ15_RS03575 (740908)	740908..741138	+	231	WP_000494239.1	YncJ family protein	-
KKZ15_RS03580 (741230)	741230..743191	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KKZ15_RS03585 (743264)	743264..743800	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KKZ15_RS03590 (743892)	743892..745067	+	1176	WP_001236258.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204284 WP_000813794.1 NZ_CP075716:c740686-740510 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204284 NZ_CP100691:c4288847-4288563 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT204284 WP_001270286.1 NZ_CP075716:c740464-740048 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204284 NZ_CP100691:c4289079-4288837 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• PDB: 6HPB