Detailed information of TA system TA204265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 77005..77527
• Replicon: plasmid pSTEC2018_607-b
• Accession: NZ_CP075699
• Organism: Escherichia coli strain STEC2018-607

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PVW2
• Locus tag: JFX52_RS26490
• Protein ID: WP_001681795.1
• Coordinates: 77246..77527 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1Q9V6
• Locus tag: JFX52_RS26485
• Protein ID: WP_001681796.1
• Coordinates: 77005..77256 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX52_RS26460 (JFX52_26460)	72421..73128	-	708	WP_001114349.1	A24 family peptidase	-
JFX52_RS26465 (JFX52_26465)	73088..73570	-	483	WP_021561187.1	lytic transglycosylase domain-containing protein	-
JFX52_RS26470 (JFX52_26470)	73618..74154	-	537	WP_001011500.1	type 4 pilus major pilin	-
JFX52_RS26475 (JFX52_26475)	74204..75304	-	1101	WP_001211427.1	type II secretion system F family protein	-
JFX52_RS26480 (JFX52_26480)	75306..76814	-	1509	WP_012605185.1	ATPase, T2SS/T4P/T4SS family	-
JFX52_RS26485 (JFX52_26485)	77005..77256	+	252	WP_001681796.1	hypothetical protein	Antitoxin
JFX52_RS26490 (JFX52_26490)	77246..77527	+	282	WP_001681795.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX52_RS26495 (JFX52_26495)	77566..77784	-	219	WP_175125356.1	hypothetical protein	-
JFX52_RS26500 (JFX52_26500)	77837..78289	-	453	WP_001681794.1	type IV pilus biogenesis protein PilP	-
JFX52_RS26505 (JFX52_26505)	78279..79574	-	1296	WP_077881449.1	type 4b pilus protein PilO2	-
JFX52_RS26510 (JFX52_26510)	79595..81214	-	1620	WP_042014174.1	PilN family type IVB pilus formation outer membrane protein	-
JFX52_RS26515 (JFX52_26515)	81245..81682	-	438	WP_001681791.1	type IV pilus biogenesis protein PilM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..90123	90123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11122.01 Da
• Isoelectric Point: 10.6249

>T204265 WP_001681795.1 NZ_CP075699:77246-77527 [Escherichia coli]
MTYELEFERRALKEWHKLGHTVREQFKKKLSERLKNPRVPAARLHGHTDRYKIKLRASGYRLVYQVIDEKIVLLVIAVGK
RESSEVYQMADIR

Nucleotide

• Length: 282 bp

>T204265 NZ_CP100689:835108-835506 [Salmonella enterica subsp. enterica serovar Newport]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9124.33 Da
• Isoelectric Point: 4.0610

>AT204265 WP_001681796.1 NZ_CP075699:77005-77256 [Escherichia coli]
MSYQILTTTAASITELKKNPMGTVAEGEGGAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVKVSL
DDL

Nucleotide

• Length: 252 bp

>AT204265 NZ_CP100689:834881-835108 [Salmonella enterica subsp. enterica serovar Newport]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: S1PVW2

Antitoxin

• AlphaFold DB: S1Q9V6