Detailed information of TA system TA204264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1778..2337
• Replicon: plasmid pSTEC2018_607-b
• Accession: NZ_CP075699
• Organism: Escherichia coli strain STEC2018-607

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: JFX52_RS26040
• Protein ID: WP_000421257.1
• Coordinates: 2062..2337 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: JFX52_RS26035
• Protein ID: WP_001178087.1
• Coordinates: 1778..2062 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX52_RS26030 (JFX52_26030)	1..873	+	873	WP_198777253.1	incFII family plasmid replication initiator RepA	-
JFX52_RS26035 (JFX52_26035)	1778..2062	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
JFX52_RS26040 (JFX52_26040)	2062..2337	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX52_RS26045 (JFX52_26045)	2443..2646	+	204	Protein_3	hypothetical protein	-
JFX52_RS26050 (JFX52_26050)	2915..3181	-	267	WP_000627831.1	hypothetical protein	-
JFX52_RS26055 (JFX52_26055)	3319..3921	-	603	WP_000517694.1	ProQ/FINO family protein	-
JFX52_RS26060 (JFX52_26060)	4275..5621	+	1347	WP_001132021.1	protein YagA	-
JFX52_RS26065 (JFX52_26065)	6220..6522	-	303	WP_001245884.1	hypothetical protein	-
JFX52_RS26070 (JFX52_26070)	6519..7145	-	627	WP_000587689.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..90123	90123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T204264 WP_000421257.1 NZ_CP075699:2062-2337 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T204264 NZ_CP100689:824241-824654 [Salmonella enterica subsp. enterica serovar Newport]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT204264 WP_001178087.1 NZ_CP075699:1778-2062 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT204264 NZ_CP100689:823994-824260 [Salmonella enterica subsp. enterica serovar Newport]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAATTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625