Detailed information of TA system TA204251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4113381..4114006
• Replicon: chromosome
• Accession: NZ_CP075697
• Organism: Escherichia coli strain STEC2018-607

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFX52_RS20005
• Protein ID: WP_000911330.1
• Coordinates: 4113608..4114006 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JFX52_RS20000
• Protein ID: WP_000450524.1
• Coordinates: 4113381..4113608 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX52_RS19975 (4109184)	4109184..4109654	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
JFX52_RS19980 (4109654)	4109654..4110226	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JFX52_RS19985 (4110372)	4110372..4111250	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JFX52_RS19990 (4111267)	4111267..4112301	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
JFX52_RS19995 (4112514)	4112514..4113227	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JFX52_RS20000 (4113381)	4113381..4113608	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JFX52_RS20005 (4113608)	4113608..4114006	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFX52_RS20010 (4114153)	4114153..4115016	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
JFX52_RS20015 (4115031)	4115031..4117046	+	2016	WP_032330806.1	tRNA cytosine(34) acetyltransferase TmcA	-
JFX52_RS20020 (4117120)	4117120..4117818	+	699	WP_000679812.1	esterase	-
JFX52_RS20025 (4117928)	4117928..4118128	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T204251 WP_000911330.1 NZ_CP075697:4113608-4114006 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T204251 NZ_CP100685:213667-214152 [Salmonella enterica subsp. enterica serovar Goldcoast]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT204251 WP_000450524.1 NZ_CP075697:4113381-4113608 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT204251 NZ_CP100685:213392-213679 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATTAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33