Detailed information of TA system TA020425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1014694..1015313
• Replicon: chromosome
• Accession: NC_002755
• Organism: Mycobacterium tuberculosis CDC1551

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MT_RS04785
• Protein ID: WP_003404726.1
• Coordinates: 1014879..1015313 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: MT_RS04780
• Protein ID: WP_003898641.1
• Coordinates: 1014694..1014873 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT_RS04770	1010167..1011747	+	1581	WP_003909313.1	serine hydrolase	-
MT_RS04775	1011744..1014137	+	2394	WP_003917373.1	cation-translocating P-type ATPase	-
MT_RS22495	1014410..1014568	+	159	WP_003404720.1	hypothetical protein	-
MT_RS04780	1014694..1014873	+	180	WP_003898641.1	antitoxin	Antitoxin
MT_RS04785	1014879..1015313	+	435	WP_003404726.1	SRPBCC family protein	Toxin
MT_RS04790	1015411..1016184	+	774	WP_003404735.1	VOC family protein	-
MT_RS04795	1016249..1016698	+	450	WP_003404738.1	hypothetical protein	-
MT_RS04800	1016833..1017063	-	231	WP_003898642.1	hypothetical protein	-
MT_RS04805	1017230..1018738	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
MT_RS04810	1018740..1019978	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T20425 WP_003404726.1 NC_002755:1014879-1015313 [Mycobacterium tuberculosis CDC1551]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T20425 NC_002755:1014879-1015313 [Mycobacterium tuberculosis CDC1551]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT20425 WP_003898641.1 NC_002755:1014694-1014873 [Mycobacterium tuberculosis CDC1551]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT20425 NC_002755:1014694-1014873 [Mycobacterium tuberculosis CDC1551]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5