Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3668798..3669452 Replicon chromosome
Accession NZ_CP075697
Organism Escherichia coli strain STEC2018-607

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag JFX52_RS17860 Protein ID WP_062851108.1
Coordinates 3669045..3669452 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JFX52_RS17855 Protein ID WP_000354046.1
Coordinates 3668798..3669064 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX52_RS17830 (3663967) 3663967..3664710 + 744 WP_001436959.1 SDR family oxidoreductase -
JFX52_RS17835 (3664767) 3664767..3666200 - 1434 WP_001342496.1 6-phospho-beta-glucosidase BglA -
JFX52_RS17840 (3666245) 3666245..3666556 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
JFX52_RS17845 (3666720) 3666720..3667379 + 660 WP_000250274.1 hemolysin III family protein -
JFX52_RS17850 (3667575) 3667575..3668555 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JFX52_RS17855 (3668798) 3668798..3669064 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JFX52_RS17860 (3669045) 3669045..3669452 + 408 WP_062851108.1 protein YgfX Toxin
JFX52_RS17865 (3669492) 3669492..3670013 - 522 WP_001055874.1 flavodoxin FldB -
JFX52_RS17870 (3670125) 3670125..3671021 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JFX52_RS17875 (3671046) 3671046..3671756 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JFX52_RS17880 (3671762) 3671762..3673495 + 1734 WP_000813168.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15989.88 Da        Isoelectric Point: 11.5202

>T204249 WP_062851108.1 NZ_CP075697:3669045-3669452 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIGKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204249 NZ_CP100682:4452617-4452928 [Salmonella enterica subsp. enterica serovar Goldcoast]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGATAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204249 WP_000354046.1 NZ_CP075697:3668798-3669064 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204249 NZ_CP100682:4452925-4453371 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References