Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3041059..3041645 Replicon chromosome
Accession NZ_CP075697
Organism Escherichia coli strain STEC2018-607

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag JFX52_RS14700 Protein ID WP_042014162.1
Coordinates 3041059..3041289 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JFX52_RS14705 Protein ID WP_000593555.1
Coordinates 3041286..3041645 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX52_RS14690 (3037096) 3037096..3039831 + 2736 WP_062851013.1 ribosome-associated ATPase/putative transporter RbbA -
JFX52_RS14695 (3039831) 3039831..3040955 + 1125 WP_062859323.1 ABC transporter permease -
JFX52_RS14700 (3041059) 3041059..3041289 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
JFX52_RS14705 (3041286) 3041286..3041645 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JFX52_RS14710 (3041765) 3041765..3042166 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
JFX52_RS14715 (3042172) 3042172..3042978 - 807 WP_032331057.1 nickel import ATP-binding protein NikE -
JFX52_RS14720 (3042975) 3042975..3043739 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
JFX52_RS14725 (3043739) 3043739..3044572 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
JFX52_RS14730 (3044569) 3044569..3045513 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T204247 WP_042014162.1 NZ_CP075697:3041059-3041289 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T204247 NZ_CP100682:c3998426-3998142 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGACGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204247 WP_000593555.1 NZ_CP075697:3041286-3041645 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204247 NZ_CP100682:c3998658-3998416 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References