Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2178697..2179194 Replicon chromosome
Accession NZ_CP075697
Organism Escherichia coli strain STEC2018-607

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JFX52_RS10655 Protein ID WP_001173343.1
Coordinates 2178697..2178870 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JFX52_RS10660 Protein ID WP_000398619.1
Coordinates 2178898..2179194 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX52_RS10645 (2175367) 2175367..2176824 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
JFX52_RS10650 (2177061) 2177061..2178578 + 1518 WP_001295074.1 lysine--tRNA ligase -
JFX52_RS10655 (2178697) 2178697..2178870 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JFX52_RS10660 (2178898) 2178898..2179194 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JFX52_RS10665 (2179421) 2179421..2179693 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JFX52_RS10670 (2179705) 2179705..2179935 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
JFX52_RS10675 (2180116) 2180116..2181747 + 1632 WP_001216487.1 sensor histidine kinase -
JFX52_RS10680 (2181744) 2181744..2182463 + 720 WP_032331011.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T204244 WP_001173343.1 NZ_CP075697:c2178870-2178697 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T204244 NZ_CP100682:1967200-1967303 [Salmonella enterica subsp. enterica serovar Goldcoast]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT204244 WP_000398619.1 NZ_CP075697:c2179194-2178898 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT204244 NZ_CP100682:c1967305-1967162 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References